Comparison of Carbapenem Resistance Detected by the BD Phoenix Automated System in Enterobacteriaceae Isolates with E-Test Method
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Mehmet Celik 1 * , Mahmut Sunnetcioglu 2 , Huseyin Guducuoglu 3 , Yusuf Arslan 4 , Sumeyye Akyuz 5 , Ali Irfan Baran 2
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1 Infectious Diseases and Clinical Microbiology Department, Medical Faculty Hospital, Harran University, Sanliurfa, TURKEY2 Infectious Diseases and Clinical Microbiology Department, Medical Faculty, Van Yuzuncu Yil University, Van, TURKEY3 Medical Microbiology Department, Medical Faculty, Van Yuzuncu Yil University, Van, TURKEY4 Infectious Diseases and Clinical Microbiology Department, Batman Training and Research Hospital, Batman, TURKEY5 Medical Microbiology Department, Mengucek Gazi Training and Research Hospital, Erzincan University, Erzincan, TURKEY* Corresponding Author

Abstract

Objective: Automatic identification and antimicrobial susceptibility systems are frequently used to identify clinical isolates in hospitalized patients, but mistakes in these systems can lead to potentially devastating treatment failures for patients. Therefore, the ‘‘Centers for Disease Control and Prevention (CDC)’’ recommends confirming all Carbapenem-resistant and low-susceptibility isolates with a different method. The aim of this study is to compare the Carbapenem susceptibility results of isolates reported as Carbapenem-resistant Enterobacteriaceae according to the BD Phoenix 100 automated system with the E-test method.
Materials and Methods: The study included 70 strains of Carbapenem-resistant Enterobacteriaceae members which were isolated and grown from several types of clinical samples in the Medical Microbiology Laboratory. Conventional methods (Gram stain, negative oxidase test) and the BD Phoenix 100 automated system were used to identify the isolates. The susceptibility of all strains to imipenem, ertapenem and meropenem was investigated by E-test method. Automated system results and E-test results were compared.
Results: The frequency distribution of all isolated bacterial strains comprised K. pneumoniae in 56 (80%) of the samples included in the study. The automated system test results were correlated with the results of the E-test at a rate of 96.1 % for the imipenem-resistant strains, 84.3% for the meropenem-resistant strains, 84.1% for the ertapenem-resistant strains
Conclusions: Automated systems are frequently used in microbiology laboratories to identify isolates. However, automated systems can show a high error rate against some antimicrobials. For this reason, comparing the results of automated system test results with tests such as E-test is very important to prevent both treatment failures and inappropriate antibiotic use that may occur on a patient basis.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 19, Issue 3, June 2022, Article No: em362

https://doi.org/10.29333/ejgm/11672

Publication date: 02 Feb 2022

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Article Downloads: 1598

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