Evaluation of cytomegalovirus DNA in periymphatic fluid in patients with sensorineural hearing loss using PCR technique
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Nader Saki 1, Ali Ghazipur 1, Manuchehr Makvandi 2, Amit Verma 3, Soheila Nikakhlagh 1, Niloofar Neisi 4, Soheila Rashidimehr 5 *
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1 Associate Professor of Otolaryngology. Hearing Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran2 Professor of Virology. Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran3 Specialist Otolaryngology. Delhi, India4 PhD of Virology. Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran5 Resident of Otolaryngology. Hearing Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran* Corresponding Author

Abstract

Purpose:
Congenital cytomegalovirus (CMV) infection is the most common congenital infection in developing countries with sensorineural hearing loss (SNHL) being the most important of its long-term sequelae. The aim of this research was to evaluate the amount of CMV DNA in perilymph of patients with SNHL undergoing cochlear implant (CI) procedure by Polymerase chain reaction (PCR) technique.

Materials and Methods:
This cross-sectional and prospective study was conducted in a group of patients with ages ranging from 1 to 7 years. The perilymph was extracted preoperatively using a Yale spinal needle after opening of the round window. 25 microliters of perilymph was extracted and stored at -80 degrees centigrade. PCR technique was employed to detect CMV DNA. The data was collected and statistically analyzed.

Results:
A sample size of 41 patients with bilateral profound SNHL was selected. After cochlear implantation, CMV DNA evaluation was performed which was found positive in 18 patients (43.9%) and negative in 23 patients (56.09%) with a statistically significant relationship. No statistical relationship was found with gender, age and other comorbid disorders. 3-6 months’ follow-up was individually performed to compare Categories of Auditory Performance (CAP) and Speech Intelligibility Rating (SIR) tests in CMV DNA positive and negative patients, yielding different results which were statistically insignificant.

Conclusion:
The early detection of CMV infection with real time PCR technique enables us to perform viral screening tests in patients with SNHL.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 16, Issue 2, April 2019, Article No: em113

https://doi.org/10.29333/ejgm/94221

Publication date: 17 Apr 2019

Online publication date: 12 Aug 2018

Article Views: 1823

Article Downloads: 1405

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