Exploration of the Beta-Actin DNA Integrity Index as Early Genetic Marker of Presence of Breast Cancer
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Wafaa Hussien Habeeb 1, Ahmed AbdulJabbar Suleiman 2 * , Haitham Tafash Al-Hitawee 3
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1 Department of Biology, College of Science, University of Anbar, Ramadi, IRAQ2 Department of Biotechnology, College of Science, University of Anbar, Ramadi, IRAQ3 College of Medicine, University of Falluja, Anbar, IRAQ* Corresponding Author

Abstract

Background: Circulating cell-free DNA (cfDNA) and its integrity index can be a fast and non-invasive (liquid biopsy) biomarker, this provides significant additional data for diagnosis, prognosis and therapy stratification in cancer patient.
Methods: The circulating tumor DNA (ctDNA) concentration and integrity was investigated in the plasma from patients with breast cancer by a quantitative polymerase chain reaction (qPCR) and their diagnostic value for breast cancer etiology was evaluated Plasma samples were collected from 55 patients 40 patients with breast cancer, 5 patients each with other type of cancer (ovarian cancer, colon cancer, stomach cancer) and 20 healthy controls. Real-time PCR of β-actin gene were investigated using two primer sets (400 and 100bp) to amplify different DNA fragment lengths. The DNA integrity index was calculated as the ratio of q-PCR results of β-actin 400bp/100.
Results: In all cancer patients the DNA concentrations were significantly higher p<0.0001, than those of the control group. The plasma DNA integrity was statistically significantly lower in breast cancer and colon cancer than the control groups (p<0.001).
Conclusion: The plasma DNA concentration and integration test can serve as a new diagnostic marker for detection and monitoring of patients with breast cancer and colon cancer.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 17, Issue 2, April 2020, Article No: em188

https://doi.org/10.29333/ejgm/7618

Publication date: 15 Feb 2020

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Article Downloads: 1963

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