Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia

Omar S. El-Masry

doi:10.29333/ejgm/12939

Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia

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Omar S. El-Masry ¹ ^*

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¹ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam, SAUDI ARABIA^* Corresponding Author

Abstract

Chronic myeloid leukemia (CML) is a common blood malignancy awaiting decisive cure. Understanding the genomic nature of the disease could help to improve treatment strategies and introduce novel therapeutic targets. The purpose of this study was to sequence the entire transcriptome of a cohort of CML patients to understand differences in gene expression profiles and gene variants compared to healthy controls. RNA was extracted from 10 CML and four healthy control subjects and sequenced by the DNBSEQ platform. Differential gene expression was evaluated and confirmed by the q-RT-PCR technique. Gene variants were also analyzed. The results showed that COX7B and COX7C were upregulated in CML patients than in controls; this was confirmed by quantitative PCR. In addition, novel single nucleotide and insertion/deletion variants were also found in both genes. In conclusion, the results of this study recommend further exploration of COX7B and COX7C and their novel variants in myeloid leukemogenesis.

Keywords

CML
COX7B
COX7C
RNA sequencing

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 20, Issue 3, June 2023, Article No: em468

https://doi.org/10.29333/ejgm/12939

Publication date: 01 May 2023

Online publication date: 05 Feb 2023

Article Views: 1004

Article Downloads: 927

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How to cite this article

Vancouver

El-Masry OS. Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia. ELECTRON J GEN MED. 2023;20(3):em468. https://doi.org/10.29333/ejgm/12939

APA

El-Masry, O. S. (2023). Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia. Electronic Journal of General Medicine, 20(3), em468. https://doi.org/10.29333/ejgm/12939

AMA

El-Masry OS. Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia. ELECTRON J GEN MED. 2023;20(3), em468. https://doi.org/10.29333/ejgm/12939

Chicago

El-Masry, Omar S.. "Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia". Electronic Journal of General Medicine 2023 20 no. 3 (2023): em468. https://doi.org/10.29333/ejgm/12939

Harvard

El-Masry, O. S. (2023). Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia. Electronic Journal of General Medicine, 20(3), em468. https://doi.org/10.29333/ejgm/12939

MLA

El-Masry, Omar S. "Gene expression profile and presentation of novel gene variants of COX7B and COX7C in a cohort of patients with chronic myeloid leukemia". Electronic Journal of General Medicine, vol. 20, no. 3, 2023, em468. https://doi.org/10.29333/ejgm/12939