REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM

Dai Yong; Xu Zhuojia; Li Tiyuan

doi:10.29333/ejgm/82389

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Dai Yong ¹ ^* , Xu Zhuojia ¹, Li Tiyuan ¹

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¹ 2nd Affiliated Hospital of Medical College of Jinan University, Shenzhen People’s Hospital, China^* Corresponding Author

Abstract

Aim: Future prospects for gene therapy of renal anemia involve expression of the EPO transgene. Control of gene expression is important to gene therapy for purposes of both dosing and safety. We tested the effects of rhEPO gene expression under the tetracycline gene expression system’s regulating and controlling. Methods: Cultured rat primary skeletal muscle cells for gene delivery use; Inserted the hEPO cDNA in the plasmid pTRE2hyg; Rat primary skeletal muscle cells were engineered for Dox-inducible and skeletal muscle-specific expression of rhEPO cDNA by co-transfection pTRE2hyg-EPO and Tet-ON plasmids. Results: EPO could be secreted by the transfected positive cells. EPO expression depended on the presence of doxycycline, and the EPO secretion was increased parallel to dose of doxycycline. Conclusion: Tetracycline gene expression system provided a suitable control system for sustained and regulated expression of a desired gene.

Keywords

EPO
Dox
Gene therapy
Skeletal Muscle cells

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Article Type: Original Article

EUR J GEN MED, Volume 3, Issue 3, July 2006, 108-115

https://doi.org/10.29333/ejgm/82389

Publication date: 15 Jul 2006

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Vancouver

Yong D, Zhuojia X, Tiyuan L. REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM. EUR J GEN MED. 2006;3(3):108-15. https://doi.org/10.29333/ejgm/82389

APA

Yong, D., Zhuojia, X., & Tiyuan, L. (2006). REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM. European Journal of General Medicine, 3(3), 108-115. https://doi.org/10.29333/ejgm/82389

AMA

Yong D, Zhuojia X, Tiyuan L. REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM. EUR J GEN MED. 2006;3(3), 108-115. https://doi.org/10.29333/ejgm/82389

Chicago

Yong, Dai, Xu Zhuojia, and Li Tiyuan. "REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM". European Journal of General Medicine 2006 3 no. 3 (2006): 108-115. https://doi.org/10.29333/ejgm/82389

Harvard

Yong, D., Zhuojia, X., and Tiyuan, L. (2006). REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM. European Journal of General Medicine, 3(3), pp. 108-115. https://doi.org/10.29333/ejgm/82389

MLA

Yong, Dai et al. "REGULATION OF ERYTHROPOIETIN EXPRESSION BY DOXYCYCLINE IN RAT PRIMARY SKELETAL MUSCLE CELLS FOLLOWING TRANSDUCTION BY TETRACYCLINE GENE EXPRESSION SYSTEM". European Journal of General Medicine, vol. 3, no. 3, 2006, pp. 108-115. https://doi.org/10.29333/ejgm/82389

Electronic Journal of General Medicine

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