Screening of Feijoa (Acca Sellowiana (O. Berg) Burret) Fruit Effect on Proliferation and Apoptosis using Bone Marrow derived Stem Cells Model
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Hosein Rasekh 1, Davood Mehrabani 2 3 * , Mehdi Hosseini Farahi 1, Seyed Jalil Masoumi 4, Jason P. Acker 3 5
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1 Islamic Azad University, Yasooj, IRAN2 Shiraz University of Medical Sciences, Shiraz, IRAN3 University of Alberta, Edmonton, AB, CANADA4 School of Nutrition and Food Sciences, Shiraz, IRAN5 Canadian Blood Services, Edmonton, AB, CANADA* Corresponding Author

Abstract

Background and Objective: Curative properties of Feijoa sellowiana Bert. (Myrtaceae, Acca sellowiana), have been often claimed, while investigations into details of its bioactivities are still lacking. This study investigated the effect of acetonic extract of Feijoa (Acca sellowiana (O. Berg) Burret) fruit on proliferation and apoptosis of bone marrow derived stem cells.
Methodology: Human bone marrow stem cells (hBMSCs) were characterized before experiments. MTT Assay was performed to determine the toxicity of Feijoa. The growth kinetics was also investigated until 7 days. Real time PCR assessed the expression of apoptosis genes.
Results: In MTT assay, Feijoa at doses ≤ 5 ng/ml did not show any cytotoxic effect on hBMSCs and increased the cell proliferation until day 4th followed by a decrease until day 7th. Population doubling time (PDT) decreased until day 4th followed by an increase until day 7th. A significant increase in expression of Bax and decrease Bcl-2 expression were recorded on day 7th.
Conclusion: The antioxidant, anti-inflammatory and modulatory activities of Feijoa can explain the increasing effect on cell proliferation till day 4th, but the apoptotic activity of Feijoa noted after four days is reported for the first time denoting to the short term antioxidant, anti-inflammatory and modulatory properties of Feijoa that should be considered for curative activity of this fruit.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 18, Issue 1, February 2021, Article No: em269

https://doi.org/10.29333/ejgm/8458

Publication date: 21 Oct 2020

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