The gene expression and protein profiles of ADAMTS and TIMP in human chondrosarcoma cell lines induced by insulin: The potential mechanisms for skeletal and articular abnormalities in diabetes
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Sumeyya Akyol 1 2 * , Zehra Karagoz 3, Nuran Dingil Inan 4, Ilknur Butun 5, Ismail Benli 5, Kadir Demircan 2, Muhammet Ramazan Yigitoglu 6, Omer Akyol 7 8 , Semsettin Sahin 5, Huseyin Ozyurt 5
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1 Beaumont Health System-Research Institute, Royal Oak, MI, USA2 Department of Medical Biology, Faculty of Medicine, Turgut Ozal University, Ankara, Turkey3 Ankara University Biotechnology Institute, Ankara, Turkey4 Biochemist, 7675 Oaks Lane, Beaumont, TX, USA5 Department of Medical Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey6 Department of Medical Biochemistry, Faculty of Medicine, Turgut Ozal University, Ankara, Turkey7 Michigan Math and Science Academy, Warren, MI, USA8 Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey* Corresponding Author

Abstract

Background:
The delay in wound healing, decrease in the long bones resilience to fracture, and delay in fracture healing are among common complications DM patients, and they still remain as challenging issues to be solved. The mechanism has not been fully understood yet, but high sugar and/or insulin deficiency or unresponsiveness to insulin in blood are potential causes to blame. Extracellular matrix degradation/remodeling is one of the important mechanisms whereby cell differentiation, bone remodeling and wound repair can be regulated. ADAMTS proteins play important roles in cartilage/bone metabolism. This study aimed to determine whether ADAMTS/TIMP proteins were affected by insulin application in OUMS-27 cells.

Material and methods:
OUMS-27 cells were induced by 10μg/mL insulin for 1,3,7,and 11days. Cells were harvested, mRNA and protein extractions were performed. Total mRNA and cDNA levels were measured by qRT-PCR and protein levels were detected by WB.

Results:
ADAMTS1,5, and 7 levels were significantly decreased, while TIMP-3 levels were detected increased (mRNA/protein concentrations).

Conclusions:
Pathologies and disturbances of cartilage/bone metabolism, delayed fracture healing in particular, in patients with DM may result from insulin deficiency. ADAMTS genes that play a role in healing process are increased during insulin deficiency, which consequently interrupts healing process by causing cartilage ECM degradation.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 17, Issue 1, February 2020, Article No: em186

https://doi.org/10.29333/ejgm/112767

Publication date: 16 Jan 2020

Article Views: 2009

Article Downloads: 1708

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