Effect of hydroalcoholic extract of Cannabis (Cannabis sativa L.) on morphology and the process of human adipose-drived mesenchymal stem cell growth
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Mehregan Jamshidi 1 2, Seyed Ebrahim Hosseini 2 * , Davood Mehrabani 3, Masoud Amini 4
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1 Department of Biology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran2 Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.3 Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.4 Laparascopy Research Center, Department of Surgery Shiraz University of Medical Science, Shiraz, Iran* Corresponding Author

Abstract

Background:
Today, cannabis sativa L. is the most consuming drug trafficking plant in the world after nicotine and alcohol.

Aims:
Given the properties of cannabis and psychoactive compounds of this opiate drug, including hashish, as well as the importance of human adipose-derived mesenchymal stem cells in the restoration and regeneration of organs and its application in tissue transplantation and tissue engineering, the current paper investigates the effect of hashish (hydroalcoholic extract of cannabis) on the morphology and growth process of human adiposed-drived mesenchymal stem cells in vitro.

Methods:
Human adipose-drived mesenchymal stem cells were extracted from the abdominal fat by the enzyme digestion of the patient’s abdominal region. MTT test was performed to determine the best concentration of cannabis extract. Cells were treated for seven days at 100 ng / ml and 1000 ng / ml, and propagation, growth and viability of the cells were evaluated.

Results:
The results show that cannabis extract reduces the proliferation of human adipose-drived mesenchymal stem cells as time-dependent. Also, the effect of cannabis extract on mesenchymal cells depends on concentrations. High concentrations have a greater negative effect on the growth of the cells. The results of this study showed that the morphology of human adipose-drived mesenchymal stem cells did not change after exposure to cannabis extract.

Conclusion:
Although in the first days, the growth process is elevated and the cells are in the euphoric phase, apoptosis increases with time as consumption increases due to toxicity. The effects of cannabis on fat cells indicate a decrease in the volume of adipose tissue and, consequently, in the consumer’s slimming.

Keywords

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 15, Issue 3, June 2018, Article No: em31

https://doi.org/10.29333/ejgm/86194

Publication date: 03 Mar 2018

Article Views: 2338

Article Downloads: 1229

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