PCOS model: Apoptotic changes and role of vitamin D

Arfa Azhar; Zehra Naseem; Ghulam Haider; Nida Farooqui; Sabah Farhat; Rehana Rehman

doi:10.29333/ejgm/12275

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Arfa Azhar ¹ , Zehra Naseem ¹ , Ghulam Haider ¹ , Nida Farooqui ¹ , Sabah Farhat ¹ , Rehana Rehman ¹ ^*

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¹ Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, PAKISTAN^* Corresponding Author

Abstract

Aim: This research was aimed to observe apoptotic marker (caspase 3) levels in different groups after treatment with vitamin D in an animal model after inducing polycystic ovary syndrome (PCOS) with dehydroepiandrosterone (DHEA).
Materials and Methods: 30 pre-pubertal female sprague dawley dams were recruited. The animals were distributed 10 each in control, PCOS and vitamin D treated groups. In control group, 0.2 ml of sesame oil was given. PCOS group was administered DHEA by the daily dose of 6 mg/kg for 30 days. In vitamin D treated group, animals were injected 6 mg/ kg/day DHEA daily and 120 ng 1, 25(OH) 2D3/100 g subcutaneously once a week. The occurrence of programmed cell death in PCOS by apoptotic marker (caspase 3) levels.
Results: The results of this study showed significant weight gain, obesity, changes in caspase 3 levels in PCOS group as compared to control and vitamin D treated group.
Conclusion: Administration of vitamin D (120 ng 1, 25(OH) 2D3/100) reduced body weight as well as improved the caspase 3 levels in PCOS induced animals. The results support the effect of vitamin D treatment for metabolic and reproductive characteristic features in PCOS females.

Keywords

animal model
DHEA
polycystic ovary syndrome
vitamin D
caspase 3

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Original Article

ELECTRON J GEN MED, Volume 19, Issue 5, October 2022, Article No: em398

https://doi.org/10.29333/ejgm/12275

Publication date: 26 Jul 2022

Article Views: 1111

Article Downloads: 1251

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How to cite this article

Vancouver

Azhar A, Naseem Z, Haider G, Farooqui N, Farhat S, Rehman R. PCOS model: Apoptotic changes and role of vitamin D. ELECTRON J GEN MED. 2022;19(5):em398. https://doi.org/10.29333/ejgm/12275

APA

Azhar, A., Naseem, Z., Haider, G., Farooqui, N., Farhat, S., & Rehman, R. (2022). PCOS model: Apoptotic changes and role of vitamin D. Electronic Journal of General Medicine, 19(5), em398. https://doi.org/10.29333/ejgm/12275

AMA

Azhar A, Naseem Z, Haider G, Farooqui N, Farhat S, Rehman R. PCOS model: Apoptotic changes and role of vitamin D. ELECTRON J GEN MED. 2022;19(5), em398. https://doi.org/10.29333/ejgm/12275

Chicago

Azhar, Arfa, Zehra Naseem, Ghulam Haider, Nida Farooqui, Sabah Farhat, and Rehana Rehman. "PCOS model: Apoptotic changes and role of vitamin D". Electronic Journal of General Medicine 2022 19 no. 5 (2022): em398. https://doi.org/10.29333/ejgm/12275

Harvard

Azhar, A., Naseem, Z., Haider, G., Farooqui, N., Farhat, S., and Rehman, R. (2022). PCOS model: Apoptotic changes and role of vitamin D. Electronic Journal of General Medicine, 19(5), em398. https://doi.org/10.29333/ejgm/12275

MLA

Azhar, Arfa et al. "PCOS model: Apoptotic changes and role of vitamin D". Electronic Journal of General Medicine, vol. 19, no. 5, 2022, em398. https://doi.org/10.29333/ejgm/12275